Tissue Worksheets are a good introduction to histology. It is an important step towards understanding the principles of tissue culture and its role in biopharmaceutical development. The worksheet consists of eight sections. You must complete the sections and answer questions before the workbook is finalized for use.
Tissue Cells and Surgitei are the first sections of the worksheet. You will have to identify the cells used in the worksheet such as the adherent or fluid forming cells and the monolayer, sieve or endothelial cells. You will also have to determine how these cells interact with the growth medium.
Isolation of Cells is the second section. You will have to grow the cells in the medium to determine how they differentiate. Your next task is to determine how to move the cells to the culture flask.
Bio sculpture is the third section, which deals with the formation of biofilms in the culture flask. Your next task is to determine how the cells interact with the biofilm. Your next section is Cytokines which provides you with answers to the question of how cells interact with the inflammatory cytokines.
Histology is the fourth section. This section is about the development of the culture flask at different stages of the histology procedure. It also introduces the major histological issues of the flask, the culture medium, and cell collection procedures.
The Cell Mismatch and Cytokine Epitopes are the fifth and sixth sections of the worksheet respectively. These sections deal with the interactions between the cell and the immune system. They provide answers to the question of how cells can be targeted by the immune system and how epithelial cells can be removed from the culture flask.
Culture and Staining are the seventh sections that deal with the culture flask at different stages of the histology procedure. This section introduces the basic cultural requirements and the cultural requirements of the flask. You will also have to define the culture condition and culture conditions. Your next task is to study the culture conditions and to evaluate the culture conditions according to the histology techniques.
In the eighth section of the worksheet, you will have to determine how the culture and the histology work together to determine the process. Your final task is to present your findings in the histology section of the worksheet. Your answers must be concise and clear. If the answers are unclear, you should consult the online histology guide provided in the worksheet.