The DNA Base Pairing Worksheet is a useful tool to determine whether or not the DNA test will give the expected results. It can also be used to determine how reliable the test is. In order to avoid incorrect results, it is important to know the proper steps to take in order to correctly use the DNA testing kit.
To properly sequence the DNA from each sample, the base pair that is to be tested should be read correctly. The two bases do not have to be exactly the same, but one of them should be one-half the length of the other. If the base pair is not followed by the other base pair, the results of the test are useless. Therefore, it is necessary to read the Base Pairing Worksheet before the test.
The test should be used according to the instructions given on the package. This worksheet is usually based on a spreadsheet, which explains the procedure step by step. A computer with a word processing program and a scanner should be used for the test. All the information needed for the test should be put in the spreadsheet. The steps that need to be performed are listed in a table, and are divided into five groups.
For a simple test, one base pair should be read with every batch of DNA. If a person has multiple DNA samples, the base pairs are read out for each of them. However, if more than one person in the family carries the DNA, the base pairs are only read for the first person.
Two simultaneous tests are performed. In a single test, the DNA base pairs are read simultaneously from one batch. The test is repeated until the desired results are obtained. The data from the test are all entered in the DNA Base Pairing Worksheet, making it easier to determine the sequence.
Results are given on a chart. Two colors are used. The first color shows the expected results. The second color shows the actual results of the test.
It is important to understand the procedures of the DNA testing kit, so that there are no problems during the test. Instructions are included on the box.
After the DNA testing kit is used, an examination of the kit is necessary. This ensures that the testing kit is working correctly. The kit should be checked for defects and faulty parts.